|   | findkm | 
| % findkm -graph cps Find Km and Vmax for an enzyme reaction Enzyme kinetics data (application-specific) file: enztest.dat Output file [enztest.findkm]: Created findkm.ps | 
Go to the input files for this example
Go to the output files for this example
| 
   Standard (Mandatory) qualifiers:
  [-infile]            infile     Enzyme kinetics data (application-specific)
                                  file
  [-outfile]           outfile    [*.findkm] Output file name
   -graphlb            xygraph    [$EMBOSS_GRAPHICS value, or x11] Graph type
                                  (ps, hpgl, hp7470, hp7580, meta, cps, x11,
                                  tekt, tek, none, data, xterm, png, gif)
   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers:
   -[no]plot           boolean    [Y] S/V vs S
   Associated qualifiers:
   "-outfile" associated qualifiers
   -odirectory2        string     Output directory
   "-graphlb" associated qualifiers
   -gprompt            boolean    Graph prompting
   -gdesc              string     Graph description
   -gtitle             string     Graph title
   -gsubtitle          string     Graph subtitle
   -gxtitle            string     Graph x axis title
   -gytitle            string     Graph y axis title
   -goutfile           string     Output file for non interactive displays
   -gdirectory         string     Output directory
   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write standard output
   -filter             boolean    Read standard input, write standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
 | 
| Standard (Mandatory) qualifiers | Allowed values | Default | |
|---|---|---|---|
| [-infile] (Parameter 1) | Enzyme kinetics data (application-specific) file | Input file | Required | 
| [-outfile] (Parameter 2) | Output file name | Output file | <*>.findkm | 
| -graphlb | Graph type | EMBOSS has a list of known devices, including ps, hpgl, hp7470, hp7580, meta, cps, x11, tekt, tek, none, data, xterm, png, gif | EMBOSS_GRAPHICS value, or x11 | 
| Additional (Optional) qualifiers | Allowed values | Default | |
| (none) | |||
| Advanced (Unprompted) qualifiers | Allowed values | Default | |
| -[no]plot | S/V vs S | Boolean value Yes/No | Yes | 
| 0.10 2 0.20 4 0.50 9 1.00 17 2.00 29 5.00 50 10.00 67 20.00 80 50.00 91 100.00 95 | 
| ---Hanes Woolf Plot Calculations--- Slope of best fit line is a = 0.01 Coefficient in Eqn of line y = ma +b is b = 0.05 Where line cuts x axis = (-0.20, 0) Where line cuts y axis = (0, 0.05) Limit-point of graph for plot = (103.00, 1.08) Vmax = 99.70, Km = 4.894611 | 
![[findkm results]](findkm.1.findkm.gif) 
| Program name | Description | 
|---|
This application was modified by 
David Martin (dmartin © rfcgr.mrc.ac.uk)
Modified May 2000